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Getting error once after every 20 minutes idle time as Could not load file or assembly 'vjscor'

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Hi all,
help needed for below issue. getting only in DEV and UAT environment

Issue Details :  getting an assembly not loaded error for .net framework 3.5 based web site application. only every after 20 minutes of idle app pool time

Login page is throwing Script manager error as 

Source Error: 

Line 38: <form id="form1" runat="server">

Line 39:

Line 40:     <asp:ToolkitScriptManager ID="ToolkitScriptManager1" runat="server">

Line 41:     </asp:ToolkitScriptManager>

Line 42:

let me know if more details required

Error details
------------------------------------------
Could not load file or assembly 'vjscor, Version=2.0.0.0, Culture=neutral, PublicKeyToken=b03f5f7f11d50a3a' or one of its dependencies. The system cannot find the file specified.
   at System.ModuleHandle.ResolveType(RuntimeModule module, Int32 typeToken, IntPtr* typeInstArgs, Int32 typeInstCount, IntPtr* methodInstArgs, Int32 methodInstCount, ObjectHandleOnStack type)
   at System.ModuleHandle.ResolveTypeHandleInternal(RuntimeModule module, Int32 typeToken, RuntimeTypeHandle[] typeInstantiationContext, RuntimeTypeHandle[] methodInstantiationContext)
   at System.Reflection.RuntimeModule.ResolveType(Int32 metadataToken, Type[] genericTypeArguments, Type[] genericMethodArguments)
   at System.Reflection.CustomAttribute.FilterCustomAttributeRecord(CustomAttributeRecord caRecord, MetadataImport scope, Assembly& lastAptcaOkAssembly, RuntimeModule decoratedModule, MetadataToken decoratedToken, RuntimeType attributeFilterType, Boolean mustBeInheritable, Object[] attributes, IList derivedAttributes, RuntimeType& attributeType, IRuntimeMethodInfo& ctor, Boolean& ctorHasParameters, Boolean& isVarArg)
   at System.Reflection.CustomAttribute.GetCustomAttributes(RuntimeModule decoratedModule, Int32 decoratedMetadataToken, Int32 pcaCount, RuntimeType attributeFilterType, Boolean mustBeInheritable, IList derivedAttributes, Boolean isDecoratedTargetSecurityTransparent)
   at System.Reflection.CustomAttribute.GetCustomAttributes(RuntimeAssembly assembly, RuntimeType caType)
   at System.Reflection.RuntimeAssembly.GetCustomAttributes(Boolean inherit)
   at System.Web.UI.AssemblyCache.GetAjaxFrameworkAssemblyAttribute(Assembly assembly)
   at System.Web.UI.ScriptManager.get_DefaultAjaxFrameworkAssembly()
   at System.Web.UI.ScriptManager..ctor()
   at AjaxControlToolkit.ToolkitScriptManager..ctor() in C:\Users\Stephen\Documents\Repos\Superexpert\AjaxControlToolkit\Server\AjaxControlToolkit\ToolkitScriptManager\ToolkitScriptManager.cs:line 94
   at ASP.login_aspx.__BuildControlToolkitScriptManager1() in D:\Projects\AEDDO\login.aspx:line 40
   at ASP.login_aspx.__BuildControlform1() in D:\Projects\AEDDO\login.aspx:line 38
   at ASP.login_aspx.__BuildControlTree(login_aspx __ctrl) in D:\Projects\AEDDO\login.aspx:line 1
   at ASP.login_aspx.FrameworkInitialize() in D:\Projects\AEDDO\Login.aspx.vb:line 912308
   at System.Web.UI.Page.ProcessRequest(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint)
   at System.Web.UI.Page.ProcessRequest()
   at System.Web.UI.Page.ProcessRequestWithNoAssert(HttpContext context)
   at System.Web.UI.Page.ProcessRequest(HttpContext context)
   at ASP.login_aspx.ProcessRequest(HttpContext context)
   at System.Web.HttpApplication.CallHandlerExecutionStep.System.Web.HttpApplication.IExecutionStep.Execute()
   at System.Web.HttpApplication.ExecuteStep(IExecutionStep step, Boolean& completedSynchronously)


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