Ajax Tool Kit is not working using master page in Visual Studio 2013

jax Tool Kit is not working using master page, but working perfectly without using master pages.

Below is the master page html code:

<%@ Register Assembly="AjaxControlToolkit" Namespace="AjaxControlToolkit" TagPrefix="asp" %>

I have added script manager into the master page.

<asp:ScriptManagerID="ScriptManager1"runat="server"><Scripts><asp:ScriptReferenceName="MsAjaxBundle"/><asp:ScriptReferenceName="jquery"/><asp:ScriptReferenceName="bootstrap"/><asp:ScriptReferenceName="respond"/><asp:ScriptReferenceName="WebForms.js"Assembly="System.Web"Path="~/Scripts/WebForms/WebForms.js"/><asp:ScriptReferenceName="WebUIValidation.js"Assembly="System.Web"Path="~/Scripts/WebForms/WebUIValidation.js"/><asp:ScriptReferenceName="MenuStandards.js"Assembly="System.Web"Path="~/Scripts/WebForms/MenuStandards.js"/><asp:ScriptReferenceName="GridView.js"Assembly="System.Web"Path="~/Scripts/WebForms/GridView.js"/><asp:ScriptReferenceName="DetailsView.js"Assembly="System.Web"Path="~/Scripts/WebForms/DetailsView.js"/><asp:ScriptReferenceName="TreeView.js"Assembly="System.Web"Path="~/Scripts/WebForms/TreeView.js"/><asp:ScriptReferenceName="WebParts.js"Assembly="System.Web"Path="~/Scripts/WebForms/WebParts.js"/><asp:ScriptReferenceName="Focus.js"Assembly="System.Web"Path="~/Scripts/WebForms/Focus.js"/><asp:ScriptReferenceName="WebFormsBundle"/></Scripts></asp:ScriptManager>

Content page html code:

<%@RegisterAssembly="AjaxControlToolkit"Namespace="AjaxControlToolkit"TagPrefix="cc1" %>

Added script into content page:

<asp:ScriptManagerProxyrunat="server"/>

I am using toolkitmanger for Calender extender below is html design:

<asp:TextBoxID="txt_Dispdate"runat="server"Style="width:50%"CssClass="form-control"AutoPostBack="True"OnTextChanged="txt_Dispdate_TextChanged"></asp:TextBox><cc1:calendarextenderID="CalendarExtender1"runat="server"TargetControlID="txt_Dispdate"OnClientDateSelectionChanged="CheckForPastDate"Enabled="True"Format="MM/dd/yyyy"></cc1:calendarextender>

Web.config control and assemblies code:

<compilationdebug="true"targetFramework="4.5.1"><assemblies><addassembly="System.DirectoryServices, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B03F5F7F11D50A3A"/><addassembly="System.Design, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B03F5F7F11D50A3A"/><addassembly="System.Web.Extensions.Design, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31BF3856AD364E35"/><addassembly="System.Windows.Forms, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B77A5C561934E089"/></assemblies><controls><addassembly="Microsoft.AspNet.Web.Optimization.WebForms"namespace="Microsoft.AspNet.Web.Optimization.WebForms"tagPrefix="webopt"/><addtagPrefix="ajaxToolkit"assembly="AjaxControlToolkit"namespace="AjaxControlToolkit"/></controls>

When I will click in textbox nothing calendar is showing. I also tried below example.https://timscyclingblog.wordpress.com/2013/03/22/ajaxcontroltoolkit-version-7-0123-with-net-4-5/

Thank you in Advance.