AjaxControlToolkit CalendarExtender not working after publish

I am using the AjaxControlToolkit CalendarExtender in my web site and its working in the visual studio 2012 but after publish to IIS Calendar Extender control won't show !

asp.net code :

<asp:TextBoxID="txtFrom"runat="server"Width="200"></asp:TextBox><asp:ImageID="image1"runat="server"ImageUrl="~/Image/calendar.png"/><ajaxToolkit:CalendarExtenderID="Calendar1"runat="server"TargetControlID="txtFrom"Format="dd.MM.yyyy"Enabled="true"PopupButtonID="image1"CssClass="cal_Theme1"></ajaxToolkit:CalendarExtender>

Web.config :

<configuration><configSections><!-- For more information on Entity Framework configuration, visit http://go.microsoft.com/fwlink/?LinkID=237468 --><sectionname="entityFramework"type="System.Data.Entity.Internal.ConfigFile.EntityFrameworkSection, EntityFramework, Version=6.0.0.0, Culture=neutral, PublicKeyToken=b77a5c561934e089"requirePermission="false"/></configSections><connectionStrings><addname="DefaultConnection"connectionString="Data Source=(LocalDb)\v11.0;AttachDbFilename=|DataDirectory|\aspnet-RechnungProgramm-20140827102620.mdf;Initial Catalog=aspnet-RechnungProgramm-20140827102620;Integrated Security=True"providerName="System.Data.SqlClient"/><addname="RechnungConnectionString"connectionString="Data Source=(LocalDB)\v11.0;AttachDbFilename=|DataDirectory|\Rechnung.mdf;Integrated Security=True"providerName="System.Data.SqlClient"/></connectionStrings><system.web><authenticationmode="None"/><compilationdebug="true"targetFramework="4.5"><assemblies><addassembly="System.Design, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B03F5F7F11D50A3A"/><addassembly="System.Web.Extensions.Design, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31BF3856AD364E35"/><addassembly="System.Windows.Forms, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B77A5C561934E089"/></assemblies></compilation><httpRuntimetargetFramework="4.5"/><pages><namespaces><addnamespace="System.Web.Optimization"/><addnamespace="Microsoft.AspNet.Identity"/></namespaces><controls><addassembly="Microsoft.AspNet.Web.Optimization.WebForms"namespace="Microsoft.AspNet.Web.Optimization.WebForms"tagPrefix="webopt"/><addtagPrefix="ajaxToolkit"assembly="AjaxControlToolkit"namespace="AjaxControlToolkit"/></controls></pages><membership><providers><!--
          ASP.NET Membership is disabled in this template. Please visit the following link http://go.microsoft.com/fwlink/?LinkId=301889 to learn about the ASP.NET Membership support in this template
        --><clear/></providers></membership><profile><providers><!--
          ASP.NET Membership Profile is disabled in this template. Please visit the following link http://go.microsoft.com/fwlink/?LinkId=301889 to learn about the ASP.NET Membership support in this template
        --><clear/></providers></profile><roleManager><!--
            ASP.NET Membership Role is disabled in this template. Please visit the following link http://go.microsoft.com/fwlink/?LinkId=301889 to learn about the ASP.NET Membership support in this template
        --><providers><clear/></providers></roleManager><!--
            If you are deploying to a cloud environment that has multiple web server instances,
            you should change session state mode from "InProc" to "Custom". In addition,
            change the connection string named "DefaultConnection" to connect to an instance
            of SQL Server (including SQL Azure and SQL  Compact) instead of to SQL Server Express.
      --><sessionStatemode="InProc"customProvider="DefaultSessionProvider"><providers><addname="DefaultSessionProvider"type="System.Web.Providers.DefaultSessionStateProvider, System.Web.Providers, Version=1.0.0.0, Culture=neutral, PublicKeyToken=31bf3856ad364e35"connectionStringName="DefaultConnection"/></providers></sessionState></system.web><system.webServer><modules><removename="FormsAuthenticationModule"/></modules><handlers><addname="ScriptResource"preCondition="integratedMode"verb="GET,HEAD"path="ScriptResource.axd"type="System.Web.Handlers.ScriptResourceHandler, System.Web.Extensions, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31bf3856ad364e35"/></handlers></system.webServer><runtime><assemblyBindingxmlns="urn:schemas-microsoft-com:asm.v1"><dependentAssembly><assemblyIdentityname="WebGrease"culture="neutral"publicKeyToken="31bf3856ad364e35"/><bindingRedirectoldVersion="0.0.0.0-1.5.2.14234"newVersion="1.5.2.14234"/></dependentAssembly><dependentAssembly><assemblyIdentityname="Microsoft.WindowsAzure.Storage"publicKeyToken="31bf3856ad364e35"culture="neutral"/><bindingRedirectoldVersion="0.0.0.0-2.1.0.4"newVersion="2.1.0.4"/></dependentAssembly><dependentAssembly><assemblyIdentityname="HtmlAgilityPack"publicKeyToken="bd319b19eaf3b43a"culture="neutral"/><bindingRedirectoldVersion="0.0.0.0-1.4.9.0"newVersion="1.4.9.0"/></dependentAssembly></assemblyBinding></runtime><entityFramework><defaultConnectionFactorytype="System.Data.Entity.Infrastructure.LocalDbConnectionFactory, EntityFramework"><parameters><parametervalue="v11.0"/></parameters></defaultConnectionFactory><providers><providerinvariantName="System.Data.SqlClient"type="System.Data.Entity.SqlServer.SqlProviderServices, EntityFramework.SqlServer"/></providers></entityFramework></configuration>

and master page ScriptManager :

<formrunat="server"><ajaxToolkit:ToolkitScriptManagerrunat="Server"EnableScriptGlobalization="true"EnableScriptLocalization="true"ID="ScriptManager1"ScriptMode="Debug"CombineScripts="false"><Scripts><%--To learn more about bundling scripts inScriptManager see http://go.microsoft.com/fwlink/?LinkID=301884 --%><%--FrameworkScripts--%><asp:ScriptReferenceName="jquery"/><asp:ScriptReferenceName="bootstrap"/><asp:ScriptReferenceName="respond"/><asp:ScriptReferenceName="WebForms.js"Path="~/Scripts/WebForms/WebForms.js"/><asp:ScriptReferenceName="WebUIValidation.js"Path="~/Scripts/WebForms/WebUIValidation.js"/><asp:ScriptReferenceName="MenuStandards.js"Path="~/Scripts/WebForms/MenuStandards.js"/><asp:ScriptReferenceName="GridView.js"Path="~/Scripts/WebForms/GridView.js"/><asp:ScriptReferenceName="DetailsView.js"Path="~/Scripts/WebForms/DetailsView.js"/><asp:ScriptReferenceName="TreeView.js"Path="~/Scripts/WebForms/TreeView.js"/><asp:ScriptReferenceName="WebParts.js"Path="~/Scripts/WebForms/WebParts.js"/><asp:ScriptReferenceName="Focus.js"Path="~/Scripts/WebForms/Focus.js"/><asp:ScriptReferenceName="WebFormsBundle"/><%--SiteScripts--%></Scripts></ajaxToolkit:ToolkitScriptManager>

using AjaxControlToolkit.dll Version 4.5.7.1213 , and i checked AjaxControlToolkit dll was in the bin folder 

How can i fix this ?