Access is denied, AjaxControlToolkit

Hi,

I am having an acces deny error when I try to run my web application.

Could not load file or assembly 'AjaxControlToolkit, Version=1.0.20229.20821, Culture=neutral, PublicKeyToken=28f01b0e84b6d53e' or one of its dependencies. Access is denied.

I check permissions on the folder and added the AjaxControlToolkit again. 

I tried with in VS2013 and VS2012.